Composite
Part:BBa_M50034:Design
Designed by: Cale Lester, Raquel Freeman, Tristan Yeung Group: Stanford BIOE44 - S11 (2016-11-01)
U10_mKO2, IPTG inducible, KAN resistant Thermosensor Construct
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 62
Illegal PstI site found at 68 - 12INCOMPATIBLE WITH RFC[12]Illegal SpeI site found at 62
Illegal PstI site found at 68 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 49
- 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 62
Illegal PstI site found at 68 - 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 62
Illegal PstI site found at 68 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 659
Design Notes
Removed 2 BsaI restriction sites. Under 2 kb for synthesis by DNA 2.0.
Source
Bajar, B., et al. 2016. Fluorescent indicators for simultaneous reporting of all four cell cycle phases. Nature Methods.
Neupert, J., Karcher, D., and Bock, R. 2008. Design of simple synthetic RNA thermometers for temperature-controlled gene expression in Escherichia coli. Nucleic Acids Research. 36(19): e124.