Composite

Part:BBa_M50034:Design

Designed by: Cale Lester, Raquel Freeman, Tristan Yeung   Group: Stanford BIOE44 - S11   (2016-11-01)


U10_mKO2, IPTG inducible, KAN resistant Thermosensor Construct


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 62
    Illegal PstI site found at 68
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 62
    Illegal PstI site found at 68
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 49
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 62
    Illegal PstI site found at 68
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 62
    Illegal PstI site found at 68
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 659


Design Notes

Removed 2 BsaI restriction sites. Under 2 kb for synthesis by DNA 2.0.


Source

Bajar, B., et al. 2016. Fluorescent indicators for simultaneous reporting of all four cell cycle phases. Nature Methods.

Neupert, J., Karcher, D., and Bock, R. 2008. Design of simple synthetic RNA thermometers for temperature-controlled gene expression in Escherichia coli. Nucleic Acids Research. 36(19): e124.


References